FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepbur-0_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepbur-0_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11669628
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC607020.520170822925975TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG72650.059.94542746
GTATGCC77200.056.595745
CGTATGC77950.056.14219344
ATGCCGT82200.052.93791647
TCGTATG83050.052.6943443
CTCGTAT87000.049.65675742
TGCCGTC89900.048.3250448
ACGTCTG94050.047.22072215
GCCGTCT96600.044.97079549
GCACACG104150.042.8403311
ACACGTC104000.042.70022613
TCTCGTA102700.042.0984341
GTCACCT105550.042.0508329
CACACGT106700.041.58709712
CACGTCT110400.040.47826414
CGTCTGA111750.039.8679316
AGCACAC117100.038.0428810
AATCTCG115650.037.38394539
CAGTCAC120100.036.92806227
ATCTCGT117750.036.6579240
Produced by FastQC (version 0.11.2)