FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepbur-0_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepbur-0_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13347389
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC246670.18480768036355275TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33650.051.70546346
GTATGCC41050.042.64254845
CGTATGC41850.042.0789944
TCGTATG47200.037.0867943
ATGCCGT47050.036.6819547
CTCGTAT51850.033.49043742
TGCCGTC52800.033.15097848
ACTTAGG56250.031.3613732
CACTTAG58450.030.29993231
TCACTTA59000.030.01702330
GCCGTCT58600.029.74833949
TTAGGCA61750.029.2484634
TAGGCAT62250.029.24041435
AGTCACT71100.025.157628
ACGTCTG72900.024.81987615
TCTCGTA71600.024.54468341
CTTAGGC74200.024.05801433
GTCACTT75850.023.81270829
GGCATCT74700.023.66509237
AGGCATC80600.022.67077636
Produced by FastQC (version 0.11.2)