FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepbl-1_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepbl-1_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11506178
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT285440.24807542521939085TruSeq Adapter, Index 20 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164020.1425495068823027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36250.055.14267348
CGTATGC42850.046.8958146
GTATGCC44400.045.1792447
CGTGGCC44550.045.17780733
ATGCCGT45600.044.2171749
TCGTATG48250.041.64917845
GCCTTAT49000.041.14814837
ACGTGGC49100.041.13348832
TATCTCG50700.039.4967241
CACGTGG51700.039.334531
ACGTCTG53550.038.75422315
TGCCGTC52500.038.40393450
GTCACGT52800.038.18622629
CTCGTAT54650.036.9648444
GCACACG56450.036.76154711
GCCGTCT55950.036.0974951
TCACGTG56950.035.40048630
GTGGCCT57600.035.06237434
CACACGT59450.035.024512
AGTCACG58350.034.674128
Produced by FastQC (version 0.11.2)