FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepbl-1_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepbl-1_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14681968
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1746651.1896565909965204TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG198300.064.5784546
GTATGCC205450.062.82837745
CGTATGC205050.062.59409344
ATGCCGT206900.061.25017547
TCGTATG206800.060.693143
TGCCGTC211300.059.5593348
TATCTCG217850.058.65518639
CTCGTAT216000.058.60909342
GCCGTCT217000.057.13772649
CACTAGC244500.055.68652331
ACTAGCT249000.054.66813332
TAGCTTA249400.054.51009434
GTCACTA249950.054.5034829
ACGTCTG253750.054.20129415
GCTTATC249450.054.1391436
AGTCACT252500.054.13399528
TCACTAG252800.053.8711430
TCTCGTA237050.053.2702441
AGCTTAT263300.051.64906335
CACACGT267100.051.59478412
Produced by FastQC (version 0.11.2)