FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepbl-1_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepbl-1_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10208147
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC279360.2736637707117658TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34100.056.8728146
CGTATGC39100.049.87037344
GTATGCC39350.049.4633645
ATGCCGT42150.046.25877847
TCGTATG43350.044.89992543
TGCCGTC46450.042.2023648
TATCTCG48750.040.35547639
CCGATGT50100.039.89299433
ACGTCTG50850.039.5745115
GCCGTCT51150.038.39164449
GCACACG53000.038.09932711
CTCGTAT51750.037.8824142
ACACGTC54100.037.0010513
CACACGT55300.036.451812
GATGTAT60150.033.34428835
CGATGTA60550.033.12287534
CACGTCT63050.031.97105214
AGCACAC67000.030.817310
CGTCTGA66000.030.49080716
TCTCGTA66050.029.78574841
Produced by FastQC (version 0.11.2)