FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepakita_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepakita_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9181712
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT274830.29932326346110616TruSeq Adapter, Index 18 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149520.16284544755923513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC33000.057.91329633
TATGCCG34600.055.03949448
GTCCGCA36300.052.64615234
CCGCACA37250.050.9323736
ACGTCCG37850.050.49271432
GTATGCC38100.050.0760747
CGTATGC38650.049.9077446
CGCACAT39600.047.9111737
TCCGCAC39900.047.81074535
ATGCCGT41050.046.7279249
TCGTATG42500.045.05965445
ACGTCTG43700.044.92775715
GCACACG47550.041.50816311
GTCACGT46150.041.48820529
TGCCGTC46100.041.45659650
CTCGTAT46550.041.1413444
GCCGTCT48450.039.22842851
AGTCACG49600.038.95528828
CACACGT50400.038.9526812
TCACGTC51650.037.1352330
Produced by FastQC (version 0.11.2)