FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepakita_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepakita_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25976556
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1461440.5625995994234185TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG173300.061.1469546
CGTATGC183750.057.7100344
GTATGCC186750.056.89543545
ATGCCGT190550.055.6304947
TCGTATG193350.054.9532143
ACGTCTG209450.051.4469715
CTCGTAT210000.050.22857342
TGCCGTC213700.049.63635648
GCCGTCT216300.048.95633749
GCACACG219450.048.94050211
CACACGT223400.048.10687312
AGTCACG221400.048.09499728
GTCACGA223050.047.70729
CGATCAG224350.047.36633333
ACACGTC235700.045.6406913
CGTCTGA244350.044.0567116
CACGTCT246800.043.67355314
TCTCGTA249950.042.36680241
TCACGAT258300.041.45091230
ACGATCA259350.041.37899432
Produced by FastQC (version 0.11.2)