FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepakita_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepakita_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12664867
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5508074.349094230519752TruSeq Adapter, Index 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130740.10323045634825855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG596700.067.7795746
CGTATGC603550.067.00873644
GTATGCC605100.066.8531345
ATGCCGT606100.066.71603447
TCGTATG608100.066.5659843
ACGTCTG612000.066.5567715
GCACACG615600.066.3693311
TGCCGTC612100.066.0675248
CACACGT619250.065.9727512
CTCGTAT610400.065.7275342
GCCGTCT615450.065.6817849
ACACGTC624700.065.3625413
CGTCTGA629350.064.7409116
CACGTCT631850.064.56827514
AGCACAC636800.064.2971910
TCGGAAG638200.064.144673
TCACGAT626550.063.99121535
GAGCACA642350.063.8171659
TCTCGTA627800.063.59205241
CACGATC628300.063.30142636
Produced by FastQC (version 0.11.2)