FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79105239
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12742241.6107959676349628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA926150.018.33291494-95
TATCATT1318100.017.80240294-95
GTATCAT1562800.017.39292594-95
ATGCCGT79050.017.2776094
CGTATCA1853350.016.53928494-95
GTACCGT72000.016.2033586
AGTACCG90800.016.1385735
CCGTATC2164750.015.467154594-95
TACCGTG79900.015.0199387
TAGACGG55100.014.8036675
ATCATTA1458600.014.55527694-95
GCCGTAT2661250.014.02352394-95
GTCTATG131650.013.9606041
GTATAGG143950.013.46233751
CGCCGTA3167350.013.33441894-95
TACACCG90550.012.98845
ACTGTCC82650.012.9087728
TATACTG156200.012.9032875
ACCGTGA115650.012.7924648
CACTGAG145650.012.5059047
Produced by FastQC (version 0.11.5)