FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_SAL60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_SAL60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121913366
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3945100.022.53263594-95
ATCATTA5144200.022.00583594-95
TAGACGG126100.021.506635
CATTAAA2833200.021.0890294-95
GTACCGT183350.020.1793846
TATCATT6396950.020.12507694-95
TATACCG326250.019.580475
GTCTAGG322150.018.5313421
GTATCAT7671100.018.33691294-95
AGTACCG261800.018.2144225
ACTGTCC188100.017.3209928
TACCGTG233950.016.4847187
CGTATCA9220600.016.22587694-95
GTCTATG377600.015.873031
GGACTGC243300.015.7146656
CCTAGAC333400.015.1718583
TAGAGCG304100.015.0873185
GTATAAG537800.015.0365911
CCGTATC10880200.014.81591294-95
AAGACCG267800.014.7558465
Produced by FastQC (version 0.11.5)