FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_SAL15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_SAL15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90786592
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG98500.021.9881275
TCATTAA3190050.021.9471594-95
GTACCGT138600.021.7262946
CATTAAA2330850.021.1427494-95
ATCATTA4015500.020.5335994-95
TATACCG256150.019.6178285
AGTACCG193050.018.9689885
GTCTAGG249950.018.8022161
TATCATT4866850.018.17823494-95
TACCGTG173150.017.9121077
GTATCAT5702350.016.7105794-95
CCTAGAC255000.016.521323
TAGAGCG221000.016.3550555
GTCTATG278450.016.2115361
ACTGTCC145450.016.0002738
CACTGAG309300.015.6784057
ACTGAGC294000.015.4923138
GTATAAG411950.015.4495841
GGACTGC190300.015.2996436
CGTATCA6752050.015.29357594-95
Produced by FastQC (version 0.11.5)