FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_POK5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_POK5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65838623
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA2023850.020.68351694-95
ATCATTA2533100.019.6872794-95
CATTAAA1552900.019.6074594-95
TATCATT3124600.018.23479394-95
GTATCAT3771450.017.92653394-95
CGTATCA4567900.016.9116794-95
TATACCG167650.016.2049185
CCGTATC5530400.015.89096994-95
GTCTAGG155750.014.9344551
GCCGTAT6694950.014.63334694-95
GTATAAG252750.014.1149271
TAGACGG78200.014.0907985
GTATAGG265150.013.7059881
CGCCGTA7953500.013.37650994-95
GTACCGT127500.013.1125416
CTATACT297000.013.0973614
TATACTG271200.013.0473095
TAGAGCG152650.013.0057035
GGACCGT144450.012.8562286
CCTAGAC159000.012.8448683
Produced by FastQC (version 0.11.5)