FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_POK180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_POK180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101205270
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG101700.023.3506245
ATCATTA2586400.020.68929394-95
TCATTAA2076200.019.65308294-95
TATCATT3230400.019.54216294-95
GTCTAGG258550.019.4478951
GTATCAT3857900.019.29273494-95
TATACCG278950.019.2738935
GTACCGT157750.018.3055846
CGTATCA4711300.018.01408694-95
AGTACCG220550.017.3786435
CATTAAA1517650.016.98993994-95
CCGTATC5687250.016.83751594-95
GTCTATG312450.016.7476651
GGACTGC208250.016.2612516
ACTGTCC165800.016.213228
TACCGTG201900.015.9491847
CTAGACA286250.015.6962584
CCTAGAC263750.015.6307523
GTATAAG433850.015.5810461
TAGAGCG252600.015.5308825
Produced by FastQC (version 0.11.5)