FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_DHA60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_DHA60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129247752
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG140150.026.1623575
ATCATTA3769000.022.07548794-95
GTCTAGG334900.022.0304871
TCATTAA2816500.021.86942594-95
TATACCG353600.021.8672835
TATCATT4813050.021.08653394-95
GTATCAT5964500.019.95363894-95
CTAGACA377700.019.8432854
CCTAGAC341500.019.7215653
CATTAAA1999000.019.69613694-95
GTACCGT227500.019.6245256
TACACCG256900.019.5602445
ACTGTCC230150.019.4804338
GTCTATG411250.019.3284931
AGTACCG309250.018.9060615
CGTATCA7342900.018.32254294-95
ACTGAGC486100.017.948218
TAGAGCG324700.017.8309355
CACTGAG503900.017.7953117
GTGTATG474450.017.3654041
Produced by FastQC (version 0.11.5)