FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_DHA60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_DHA60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141081213
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA6332500.024.54042894-95
CATTAAA4486050.023.63172794-95
ATCATTA8468800.023.21030894-95
TATCATT10709450.020.99075194-95
TAGACGG137950.018.5575965
GTATCAT13147000.018.54975794-95
TATACCG331750.018.4113125
GTACCGT218850.018.1432236
GTCTAGG321950.017.5238041
AGTACCG288350.016.9162925
CGTATCA15849000.016.12968694-95
ATTAAAA3647600.016.0662294-95
TAGAGCG318650.015.6207555
TACCGTG276000.015.5392577
GTCTATG386650.015.4526781
ACTGTCC217900.015.2792688
CCTAGAC320850.015.114063
CACTGAG440200.015.0513617
ACTGAGC437200.014.9587578
GTATAAG496500.014.9081071
Produced by FastQC (version 0.11.5)