FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_DHA5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_DHA5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64501705
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC3632500.016.3645894-95
CATTAAA1426450.016.25841994-95
GCCGTAT4595150.016.18500994-95
TCATTAA1671700.016.1486894-95
TATACCG182500.016.0053795
CGTATCA2946200.015.53790594-95
CGCCGTA5535600.014.99723594-95
GTATCAT2522050.013.80803794-95
ATCATTA1936600.013.46250994-95
GTCTAGG171050.012.9838261
TAGACGG99150.012.454735
TATACTG282150.012.3389345
TAGAGCG176700.012.3106975
CCTAGAC169650.012.2904573
TATCATT2219000.012.270658594-95
GTATAAG250350.012.081431
GTATAGG238250.011.9564641
TCGCCGT6628650.011.79229194-95
ATTAAAA1485650.011.72750894-95
CCTATAC245100.011.2006163
Produced by FastQC (version 0.11.5)