FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_DHA5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_DHA5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69091957
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA1848300.018.95356494-95
CATTAAA1498650.018.45602294-95
ATCATTA2210350.016.70144894-95
CGTATCA3678000.016.31487394-95
GTATCAT3102000.015.99401694-95
CCGTATC4466750.015.81700794-95
TATCATT2639000.015.25453494-95
GCCGTAT5477500.015.08196294-95
CGCCGTA6557700.014.05597894-95
TATACCG177150.013.8613575
ATTAAAA1536500.011.7509294-95
TCGCCGT7978300.011.34651994-95
GTCTTAG160550.011.199641
CCTAGAC163900.011.1278483
ACTGTCC160250.011.0548628
TATACTG278250.010.7367265
CTAGACA247400.010.65492254
GTCTAGG164350.010.6223091
GTATAGA241300.010.60591
GTATAAG230550.010.5433441
Produced by FastQC (version 0.11.5)