FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_DHA180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_DHA180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156667860
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG192950.025.8708695
GTCTAGG424950.021.6719761
TATACCG487250.021.1037275
GTCTATG544900.020.1313761
TAGAGCG426750.019.1651735
ATCATTA3469000.019.14564994-95
GTACCGT305150.018.9888886
TATCATT4252900.018.8045194-95
GTATCAT5102500.018.6852894-95
CCTAGAC438700.018.5998973
TCATTAA2705650.018.46597994-95
CTAGACA530150.018.3836824
CGTATCA6130900.017.98439694-95
ACTGAGC645050.017.9359268
TACACCG343150.017.8687975
AGTACCG417350.017.8328865
CACTGAG689900.017.7960627
CCGTATC7428550.017.1936894-95
ACTGTCC301700.017.0172868
ATAGACG230600.017.0127544
Produced by FastQC (version 0.11.5)