FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_DHA180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_DHA180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103660627
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG106150.027.4727655
TATACCG299400.022.5104715
GTCTAGG264050.021.9412861
ATCATTA2788850.021.61135994-95
TCATTAA2124100.020.97430894-95
TATCATT3532300.020.8012994-95
GTACCGT166100.020.6167226
GTCTATG338600.019.9059931
GTATCAT4304650.019.89340894-95
ACTGTCC170850.019.4314168
AGTACCG236250.019.1792035
TACCGTG211500.018.8633737
CATTAAA1507500.018.66877794-95
CTAGACA306950.018.552664
TAGAGCG271900.018.498685
CACTGAG381350.018.3828587
CGTATCA5281000.018.3665294-95
CCTAGAC274450.018.2403813
TACACCG193150.018.098275
ACTGAGC363200.017.6927058
Produced by FastQC (version 0.11.5)