FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_DHA15-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_DHA15-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98576811
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTA2978300.022.46308194-95
TATCATT3846300.021.83599994-95
TAGACGG103950.021.6117735
TCATTAA2264300.021.0938994-95
GTATCAT4808750.020.9441294-95
CGTATCA5966850.018.84144894-95
GTCTAGG240900.018.740291
CATTAAA1644650.018.53406394-95
TATACCG244300.018.508335
CTAGACA259000.017.3662094
CCTAGAC250700.017.2970543
CCGTATC7247750.016.96977294-95
GTACCGT158500.016.8407276
GTCTATG287600.016.6731851
ACTGTCC166050.016.4463318
TACACCG188050.016.164435
GTATAAG374900.015.7979531
TAGAGCG243950.015.7507575
AGTACCG218300.015.5127775
ACTGAGC331600.015.5115088
Produced by FastQC (version 0.11.5)