FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_DHA15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_DHA15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67295388
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG98650.021.5691645
TATACCG251850.017.406535
GTCTAGG203850.017.2225171
CCGTATC2343000.015.92532694-95
CGTATCA1991450.015.74787594-95
TCATTAA1148800.015.5078494-95
GTATCAT1750250.015.36726794-95
ATCATTA1383550.015.33925394-95
GTCTATG261350.015.1989981
CCTAGAC198450.015.1737253
TATCATT1595800.014.74032694-95
GCCGTAT3098200.014.54356394-95
CATTAAA894100.014.54153694-95
CGCCGTA3618800.014.31403794-95
TAGAGCG222150.014.2604075
GACGGTC127550.014.0753417
ACTGTCC144250.013.8283328
ATAGACG122250.013.6367554
CTAGACA283300.013.3617864
TATACTG318800.013.0955285
Produced by FastQC (version 0.11.5)