FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_BG760-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_BG760-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56728244
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1537890.27109776216587983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG163400.019.7092511
GTCTATG201650.018.7071881
TATACCG144350.018.063645
TAGACGG80300.017.5075725
TAGAGCG219800.017.3515225
CCTAGAC152550.016.7814143
CACTGAG402200.016.7212737
GTATCAT759650.016.60228394-95
CGTATCA888400.016.49361294-95
CCGTATC1042050.016.34405794-95
GTACCGT147450.016.3310056
ACTGAGC396400.016.11498
ACTGTCC163700.016.0731168
GTATAAG186300.016.0609551
AGTACCG181750.015.94061855
TATACTG191100.015.4092235
GACAGTG215900.015.1570537
TATCATT693000.015.08242394-95
GTGTATG199750.014.9795041
CTACCAC391550.014.9442973
Produced by FastQC (version 0.11.5)