FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_BG760-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_BG760-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134308744
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA4669250.025.39936894-95
ATCATTA6362850.024.26997294-95
CATTAAA3178200.023.78174894-95
TATACCG332650.022.0165625
TAGACGG134250.022.0053715
TATCATT8140550.021.81254694-95
GTACCGT202800.021.1013136
GTCTAGG327400.020.5737111
AGTACCG279850.019.8909235
GTATCAT10022550.019.23056494-95
TAGAGCG332300.018.7094885
CTAGACA330350.018.5324024
ACTGTCC216900.018.5246248
TACCGTG259100.017.8909037
GTATAAG494150.017.8764461
CCTAGAC339700.017.7567393
GTCTATG375250.017.607981
TACACCG262200.017.0092645
GTGTATG430250.016.551181
CGTATCA12108400.016.52828294-95
Produced by FastQC (version 0.11.5)