FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_BG75D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_BG75D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134805
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAC200.002070287571.248333
CTCCGTG250.00501215656.9986659
ATATACT451.5089849E-552.776544
ATCTAAG502.783286E-547.5871431
CTAGTAC405.3717877E-447.498893
GTAACCC405.3717877E-447.498893
CTATACG502.8140184E-547.4988864
ATACTAA502.8140184E-547.4988866
CAGTACT459.5959264E-442.2212334
TATACTT459.5959264E-442.2212335
CGGAGAT459.5959264E-442.2212335
GTAATAA500.001596310238.0697141
GTGTATG500.001596310238.0697141
ACTATAC500.001610945137.999113
TCCTGTA500.001610945137.999112
CTAAGTC500.001610945137.999113
TAACCCA651.3221562E-436.53764
AGATTCC550.002571884834.5446478
TCATACA550.002571884834.5446474
AACAGAC550.002571884834.5446477
Produced by FastQC (version 0.11.5)