FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_BG75D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_BG75D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96373572
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1447210.15016668677591405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA2042300.021.15778294-95
ATCATTA2627650.020.78130394-95
TATCATT3273600.019.42067394-95
CATTAAA1524900.019.10455794-95
GTATCAT3949800.018.5550594-95
TATACCG252600.017.8436245
CGTATCA4786200.017.25505694-95
TAGACGG133250.015.9327415
CCGTATC5747600.015.89630494-95
GTATAAG328550.015.3911041
GTCTAGG259400.014.8543281
GTACCGT195700.014.610196
TAGAGCG259500.014.5322675
GCCGTAT7116250.014.39726594-95
GTCTATG292250.014.2101191
CACTGAG397200.013.9423487
CCTAGAC263250.013.9103413
ACTGAGC381900.013.8290988
AGTACCG245700.013.6473955
TATACTG363000.013.6336245
Produced by FastQC (version 0.11.5)