FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_BG7180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_BG7180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193988737
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3157570.16277079014128537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG262750.022.7581565
TATACCG526100.021.161355
TAGAGCG610150.020.6049425
GTCTAGG553350.020.5284331
GTCTATG656400.019.3782371
GTACCGT424950.019.0563896
ATCATTA3231050.018.41906294-95
GTATCAT4640250.018.38295794-95
TATCATT3919950.018.1647794-95
AGTACCG554000.018.1323745
TCATTAA2505300.017.89671594-95
CGTATCA5595050.017.87831594-95
CACTGAG1016900.017.7436087
ACTGAGC962800.017.526738
CCGTATC6878400.017.51948794-95
GTGTATG770100.017.252231
CCTAGAC554300.017.0772573
CTAGACA623800.016.7735064
GTATAAG686100.016.5495591
ACTGTCC452650.016.1899388
Produced by FastQC (version 0.11.5)