FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_BG7180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_BG7180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97159261
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1017680.10474348914613503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG118600.025.3897885
GTCTAGG290050.021.10761
TATACCG315150.019.772935
GTACCGT184350.019.2713876
GTCTATG355100.018.9824521
GTATCAT2187050.018.12450294-95
GACGGTC149800.017.7869997
CCTAGAC291600.017.770253
CGTATCA2614600.017.76701294-95
AGTACCG265300.017.6878175
CCGTATC3128150.017.68107894-95
TAGAGCG306100.017.5956085
TATCATT1941100.017.11970594-95
ATCATTA1640950.016.90488494-95
CTAGACA328250.016.8134484
GGACTGC226600.016.726216
ACTGTCC194150.016.683528
CACTGAG460400.016.2685187
TATACTG433500.016.215385
TACCGTG222450.016.1627457
Produced by FastQC (version 0.11.5)