FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_BG715-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_BG715-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104281502
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2359740.22628557843365166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG128900.025.9032635
TATACCG311250.022.3247725
GTCTAGG302200.021.3129391
GTACCGT214900.020.8635226
GTCTATG359450.020.6180731
TAGAGCG333050.020.5640145
AGTACCG298400.019.3865495
GTATCAT2424150.019.3319894-95
CTAGACA336500.019.1111264
CCTAGAC301950.018.7969253
CGTATCA2929950.018.62613594-95
TATCATT2058950.018.46123594-95
TACACCG248100.018.2247775
ACTGTCC219600.018.1888268
ATCATTA1679150.018.17404794-95
CCGTATC3535800.017.85639494-95
GTATAAG432900.017.7461451
ACTGAGC503850.017.6365458
GTGTATG414100.017.6213511
CACTGAG551450.017.5442337
Produced by FastQC (version 0.11.5)