FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n02_BG715-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n02_BG715-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154271166
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1887030.12231903400535653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA4350000.023.15969394-95
TAGACGG171350.023.0064415
TATACCG431700.022.4551735
CATTAAA3097350.021.9430894-95
ATCATTA5754850.021.66941394-95
GTACCGT274100.020.6202246
TATCATT7074650.019.49018994-95
GTCTAGG397900.019.3203681
AGTACCG377700.018.6361715
GTCTATG481700.018.4874571
TAGAGCG448450.018.4179385
TACCGTG337250.017.8856837
GTATCAT8529600.017.7099594-95
ACTGTCC288350.017.5087558
CGTATCA10267150.016.17006194-95
CACTGAG705850.015.9272067
CTAGACA449900.015.9199374
TACACCG322200.015.7140135
CCTAGAC417600.015.6159213
ACTGAGC643450.015.530058
Produced by FastQC (version 0.11.5)