FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79105239
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATG120150.018.7781471
AGTACCG83300.017.8188635
GTACCGT62950.016.9710246
TAGACGG50950.016.421945
ACTGTCC66900.016.321818
GTGTATG123800.015.7028811
GTCTAGA103100.015.2771991
CACTGAG140550.015.2016647
GTATAGG121350.014.8895561
TACCGTG78900.014.8579267
TAAGGTG107900.014.7201815
GACAGTG77300.014.6151067
TAGAGGT100650.014.0897354
ACTGTGC80650.013.9493848
GTCTTAG82100.013.9421691
TATACTG150900.013.6268475
ACCGTGA109150.013.4684958
GTCGGTC155450.013.4404568
GCACTGA78550.013.1793286
GGTCGGT158800.013.097427
Produced by FastQC (version 0.11.5)