FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_SAL60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_SAL60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121913366
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT3767050.023.94585694-95
CTTCTGC5094800.022.66805894-95
TCTGCTT2736900.021.68650894-95
TAGACGG124400.021.419925
GTACCGT184450.020.961446
TCTTCTG6739350.019.9158794-95
TATACCG332600.019.7218115
GTCTAGG314850.018.1983911
CTGCTTG1761000.018.19770894-95
AGTACCG264400.018.0003785
GTCTTCT8084200.017.9619194-95
TACCGTG235450.017.6107527
GGACTGC243300.017.0235356
GTCTATG366700.016.8171791
ACTGTCC188850.016.750158
GTATAAG534900.016.2009931
CCTAGAC340000.015.9956513
CGTCTTC9700850.015.96629394-95
TAGAGCG293800.015.6171115
GTGTATG420700.015.019951
Produced by FastQC (version 0.11.5)