FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_SAL15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_SAL15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90786592
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2997750.023.36636494-95
TAGACGG98300.022.468535
TCTGCTT2222850.021.76168894-95
CTTCTGC3928550.021.20315694-95
GTACCGT143250.020.7565386
TATACCG261150.020.47975
GTCTAGG243300.020.4063661
TACCGTG177800.018.6460447
AGTACCG200550.018.6155175
CTGCTTG1493750.018.5896394-95
TCTTCTG5027900.018.20159394-95
ACTGTCC149200.017.6042848
GTCTATG279800.016.9292981
TAGAGCG223300.016.676415
CCTAGAC260650.016.6739543
GTCTTCT5938550.016.42035994-95
ACTGAGC298300.016.4000848
TACACCG183800.016.3581225
CACTGAG319900.016.3469287
GGACTGC202700.016.2388296
Produced by FastQC (version 0.11.5)