FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_POK5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_POK5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65838623
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2061100.021.23283894-95
TCTGCTT1560650.020.01487494-95
CTTCTGC2650850.019.85296694-95
TCTTCTG3425450.018.01722794-95
CTGCTTG1096650.017.59332894-95
GTCTTCT4129550.017.38396594-95
TATACCG166650.016.8728375
CGTCTTC5076150.016.10837694-95
GTCTAGG156000.015.9586261
TGCTTGA826900.015.9160394-95
GTATAGG261000.015.6911791
CCGTCTT5968300.015.53470994-95
GCTTGAA644500.014.6786894-95
GCCGTCT7135400.014.589581594-95
CCTAGAC163250.014.2565553
TAGACGG86250.013.93263155
ACTGTCC123950.013.8714098
GTATAAG250900.013.8611491
CTAGACA217000.013.3956384
GTACCGT123900.013.2257256
Produced by FastQC (version 0.11.5)