FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_POK180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_POK180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101205270
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG105600.024.873415
CTTCTGC2435750.021.712694-95
GTCTAGG250800.021.6525521
TTCTGCT1843200.021.39262894-95
TATACCG273050.020.7004075
TCTTCTG3317350.019.27391894-95
GTCTTCT3972500.018.79302494-95
GGACTGC211200.018.3064696
CACTGAG355200.017.9582797
GTACCGT157600.017.8418376
CCTAGAC273450.017.665143
ACTGAGC346150.017.4947138
CGTCTTC4924050.017.4883194-95
TCTGCTT1414800.017.48671594-95
ACTGTCC160150.017.4089328
GTCTATG309000.017.4051741
GTATAAG436750.017.383381
TACACCG208000.017.149475
AGTACCG223800.017.021135
TAGAGCG248500.016.9348535
Produced by FastQC (version 0.11.5)