FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_DHA60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_DHA60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129247752
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG143050.028.986745
GTCTAGG333000.023.8840181
TTCTGCT2784050.022.48144194-95
CTTCTGC3802750.022.40587494-95
GTACCGT234200.021.8830366
TATACCG379550.021.136535
AGTACCG311600.021.0051025
CTAGACA392850.020.6748494
TCTTCTG5128500.020.67139894-95
CCTAGAC358050.020.3230133
TAGAGCG328700.019.825655
ACTGTCC225750.019.7348278
GTCTATG416850.019.7293821
GTCTTCT6312750.019.49730394-95
TACACCG255400.019.490075
ACTGAGC492400.019.0891238
ATAGACG172450.019.0596944
TCTGCTT2092100.018.95090994-95
CACTGAG516550.018.8127967
GACAGTG244250.018.8040757
Produced by FastQC (version 0.11.5)