FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_DHA60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_DHA60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141081213
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT6431150.025.07225294-95
TCTGCTT4562250.023.88614794-95
CTTCTGC8791650.023.32562894-95
CTGCTTG2918850.020.66838894-95
TCTTCTG11661100.020.54850694-95
TAGACGG140800.020.3082285
GTCTAGG302350.019.7367781
TATACCG335150.019.0616845
ACTGTCC208250.018.8162468
GTACCGT222200.018.3836866
GTCTTCT14142700.018.19794894-95
AGTACCG289750.017.6551115
TGCTTGA2028600.017.21282894-95
GTCTATG386150.016.9423831
TAGAGCG323400.016.3027615
GTATAAG507700.016.2550851
TACCGTG287350.016.182167
CCTAGAC339000.016.0989863
TACACCG266950.015.8179445
CGTCTTC17024400.015.76998894-95
Produced by FastQC (version 0.11.5)