FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_DHA5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_DHA5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64501705
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT1025150.015.46982394-95
GCCGTCT1035900.015.08229594-95
TGCCGTC1639400.013.98375594-95
TATACCG190600.013.8557215
TTCCGTC886050.013.65501394-95
TCCGTCT641750.013.47554194-95
GTCTAGG178150.013.2804371
CCTAGAC171700.013.0018413
TAGACGG98500.012.6822655
TAGAGCG169150.012.4677545
TATACTG286350.012.059095
GTACCGT142100.011.9999016
ACTGAGC273900.011.91323958
ATTCCGT811200.011.91089594-95
ATGCCGT1332850.011.83432894-95
TATGCCG1525250.011.6784192-93
GTCTAGA222300.011.5832041
ACTGTCC158550.011.5634178
CACTGAG269300.011.5523717
GTCTATG218950.011.47835351
Produced by FastQC (version 0.11.5)