FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_DHA5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_DHA5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69091957
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT1882600.018.72313394-95
TCTGCTT1506350.018.215294-95
CTTCTGC2284350.016.36816294-95
CTGCTTG1093600.016.1851694-95
GTCTTCT3296200.015.53090894-95
TGCTTGA839600.015.41229594-95
CCGTCTT4701600.015.39372494-95
GCTTGAA646850.015.27906694-95
CGTCTTC4107150.015.06444694-95
TCTTCTG2815600.014.87503294-95
GCCGTCT5746750.014.80822394-95
TGCCGTC6789950.013.76969494-95
TATACCG182000.012.7617925
ATGCCGT7547300.012.0716694-95
GTCTAGG164250.012.0333961
GTATAAG235250.011.8551951
CCTAGAC163300.011.7217583
GTCTTAG158300.011.4352141
GGACCGT166450.011.2431146
GTATAGG239250.011.2399591
Produced by FastQC (version 0.11.5)