FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_DHA180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_DHA180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156667860
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG192000.027.2866575
GTCTAGG429050.023.2098851
TATACCG491100.021.4423245
TAGAGCG450950.020.5602075
GTCTATG553350.019.867951
GTACCGT322450.019.7092366
AGTACCG427900.019.3588815
CCTAGAC432100.018.9948373
ACTGAGC649000.018.9913338
CACTGAG698900.018.4509437
CTTCTGC2648650.018.29131194-95
GTGTATG655800.018.2782861
ACTGTCC300200.018.2107328
GTCTTCT3955350.017.9743894-95
TCTTCTG3350900.017.80862694-95
TACACCG350950.017.7838675
GTATAAG678150.017.5287591
TATACTG660650.017.3485015
ATAGACG243550.017.2596194
GGACTGC374600.017.1682876
Produced by FastQC (version 0.11.5)