FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_DHA180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_DHA180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103660627
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG114400.029.063435
GTCTAGG268800.024.0909541
GTACCGT172900.022.7188016
TATACCG305200.022.2238125
CTTCTGC2542250.022.17073694-95
TTCTGCT1835050.021.88880394-95
GTCTATG342550.021.1095071
TAGAGCG269850.021.0867315
AGTACCG252300.020.708585
ACTGTCC165550.020.5421818
TCTTCTG3429700.020.48808594-95
CCTAGAC277350.020.3452553
GGACTGC213850.019.7010446
GTCTTCT4166500.019.4217694-95
TACCGTG224100.019.286937
TACACCG199950.019.241485
GTGTATG378150.019.235281
CTAGACA312750.019.1358664
ACCGTGA282200.018.9683538
CACTGAG392100.018.7515727
Produced by FastQC (version 0.11.5)