FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_DHA15-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_DHA15-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98576811
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG104500.023.5444955
CTTCTGC3031800.023.0289694-95
TTCTGCT2203750.022.59720294-95
GTCTAGG235600.021.557631
TCTTCTG4222100.021.34147694-95
GTACCGT165650.020.501736
GTCTTCT5230400.020.4497494-95
TCTGCTT1639950.019.12099694-95
TATACCG242350.018.9521885
ACTGTCC159400.018.7721238
CCTAGAC256100.018.7692473
CGTCTTC6486350.018.29938594-95
AGTACCG225950.018.1625695
TACCGTG204350.018.0131637
CTAGACA268950.017.9607854
GTATAAG376250.017.5650441
ACTGAGC336800.017.3739978
CACTGAG351200.017.3378967
GTCTATG280150.017.2476121
TAGAGCG250100.016.8265845
Produced by FastQC (version 0.11.5)