FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_DHA15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_DHA15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67295388
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG96900.023.8715255
GTCTAGG200600.020.6298071
TATACCG258750.018.2649175
CCTAGAC208300.017.9000973
ATAGACG117000.017.050554
TAGAGCG214300.017.04435
TTCTGCT1068450.016.93067294-95
GACGGTC122750.016.7930777
GTCTATG259900.016.6540571
CTTCTGC1312350.016.58038794-95
CTAGACA289150.015.6546984
GCCGTCT3024650.015.41903894-95
ACTGTCC151050.015.4076638
CCGTCTT2510650.015.28253494-95
GTCTTCT1856950.015.14187294-95
TCTTCTG1636550.015.08243194-95
CGTCTTC2183900.014.957746594-95
TGCCGTC3629150.014.60145594-95
GTACCGT157900.014.4990626
TATACTG319950.014.47429855
Produced by FastQC (version 0.11.5)