FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_BG760-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_BG760-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56728244
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATG200300.021.7502611
GTCTAGG168100.021.1967681
TAGACGG79650.019.9175075
TAGAGCG224550.019.5871945
CCTAGAC155200.019.3419253
TATACCG151050.018.9928655
ACTGTCC169200.018.1059538
CACTGAG430100.017.680137
GACAGTG218450.017.6767377
GTATAAG193800.017.4297811
TATACTG190800.016.9528625
ACTGAGC421550.016.7992768
AACAGAC195650.016.7264187
GTGTATG192250.016.6065351
GTACCGT162450.016.4027796
AGTACCG197300.016.2258365
GTATAGG190850.015.881981
TACACCG150950.015.8273855
CTACCAC409100.015.8248883
TATAGTG252900.015.4194415
Produced by FastQC (version 0.11.5)