FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_BG760-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_BG760-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134308744
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT4490350.026.11953294-95
TAGACGG136350.024.5240925
CTTCTGC6373300.024.2329394-95
TCTGCTT3125850.023.706294-95
GTACCGT211050.023.1132226
TATACCG336200.022.3079595
TCTTCTG8453700.021.42856694-95
TACCGTG261500.020.1429217
AGTACCG299800.020.1209055
ACTGTCC216650.019.467758
GTCTAGG338900.019.192171
GTCTATG372950.019.1215111
CTGCTTG1942200.019.05603494-95
GTCTTCT10329250.018.8531994-95
TAGAGCG334900.018.0546345
GTATAAG507500.017.8809031
TACACCG268350.017.8593225
CCTAGAC341350.017.7413043
CACTGAG506900.017.6257
ACTGAGC466500.017.491748
Produced by FastQC (version 0.11.5)