FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_BG75D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_BG75D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134805
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTA200.002070223271.2488865
ACTGGAT405.371581E-447.499263
CACAACA652.572593E-643.8454674
CTACCGA459.5955597E-442.221564
GTAGGTG651.3220953E-436.537893
CATATAC801.07068845E-535.6244433
CTATAAG702.0394992E-433.940631
GAGCTGG600.003931847431.6779231
GCAGTAG600.003938999531.6661743
GTACTGC600.003938999531.6661746
GTTATCC600.003938999531.6661743
GTGTAGG804.46355E-429.6980511
TAGGTGG804.4732672E-429.6870374
ACACTAT804.4732672E-429.6870376
GAGCAAT650.005815913429.2411581
CCTTAGG700.00835127827.1525061
TCCCGGT700.00835127827.1525062
GGGTCGT350.00838101927.14243332-33
ATATACA700.00836641427.1424334
CAAGGGA700.00836641427.1424334
Produced by FastQC (version 0.11.5)