FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_BG75D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_BG75D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96373572
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2050300.021.27404694-95
CTTCTGC2777700.020.03832294-95
TATACCG245050.018.9953825
TCTTCTG3490350.018.87788694-95
TCTGCTT1592950.018.250794-95
GTCTTCT4237250.017.85751594-95
TAGACGG126000.016.6243535
CGTCTTC5298400.016.14906594-95
GTCTAGG254550.015.8274691
AGTACCG243100.015.5135885
CCGTCTT6089250.015.48399994-95
GTACCGT196700.015.2853736
GTATAAG331950.015.1712681
CCTAGAC260050.014.8494433
TAGAGCG265000.014.5003795
GCCGTCT7290550.014.46514994-95
ACTGAGC377450.014.4458748
GTCTATG297350.014.3641671
GTGTATG346050.014.2373321
CTGCTTG1115250.014.19263494-95
Produced by FastQC (version 0.11.5)