FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_BG7180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_BG7180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193988737
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG269750.022.9258655
GTCTAGG560800.022.3918551
TATACCG529550.022.1098675
GTCTATG651400.020.7800031
TAGAGCG618250.020.3206565
GTACCGT448650.019.4904696
AGTACCG564150.018.8342175
ACTGAGC968100.018.7366378
CACTGAG1034300.018.4237427
CCTAGAC552500.018.2599053
ACTGTCC449750.018.090418
TCTTCTG4264200.017.71966694-95
TTCTGCT2604400.017.67363294-95
GTCTTCT5089200.017.60685794-95
GTGTATG775150.017.3093131
TACACCG502550.017.286645
CTTCTGC3572550.017.2621794-95
ATAGACG274350.017.2090844
GTATAAG688600.016.890611
CCGTCTT7459450.016.85377594-95
Produced by FastQC (version 0.11.5)