FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_BG7180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_BG7180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97159261
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG115550.026.5545275
GTCTAGG282050.023.0607011
GTACCGT193050.020.7657996
TATACCG316550.020.4967215
GTCTATG358700.019.7355881
AGTACCG271000.019.3672965
TAGAGCG309450.018.4497285
ACTGTCC184950.018.3361368
CCTAGAC295650.018.2023643
GACGGTC149600.018.192337
CTTCTGC1559450.018.0107194-95
GTCTTCT2312800.017.62404894-95
TCTTCTG1965500.017.55391994-95
CACTGAG461250.017.4129567
TTCTGCT1187500.017.379594-95
CGTCTTC2768900.017.1871994-95
GCCGTCT4094100.017.1603594-95
GTGTATG395950.017.1349561
CTAGACA340800.017.1288454
GGACTGC229700.017.1216416
Produced by FastQC (version 0.11.5)