FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_BG715-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_BG715-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104281502
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG139050.029.4792025
GTCTAGG306350.025.372011
TATACCG322050.023.1111555
GTCTATG369300.022.8611221
TAGAGCG338850.021.4186365
CCTAGAC308250.020.9715713
GTACCGT221500.020.9506136
CTAGACA342500.020.330564
ATAGACG166700.020.2586234
ACTGAGC518600.019.920038
CACTGAG570550.019.7712447
AGTACCG298300.019.7603425
GTGTATG428450.019.2947241
AACAGAC274650.018.9537457
TACACCG255350.018.8057755
TATACTG396100.018.8025685
ACTGTCC225850.018.5906858
GTATAAG447100.018.5536371
GACAGTG273750.018.2005887
TACCGTG276450.017.4043167
Produced by FastQC (version 0.11.5)