FastQCFastQC Report
Mon 19 Oct 2020
HVF5FDRXX_n01_BG715-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5FDRXX_n01_BG715-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154271166
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT4382650.023.62281694-95
TATACCG430550.022.1522835
CTTCTGC5963700.021.57070794-95
TCTGCTT3266700.021.2028794-95
GTACCGT284250.020.5867866
GTCTAGG409300.019.9889621
AGTACCG379500.019.4373935
TAGAGCG448550.019.4101985
TCTTCTG7574450.019.22818894-95
GTCTATG477450.018.8672811
ACTGTCC293050.018.4768148
CTGCTTG2200700.017.56554694-95
GTCTTCT9099050.017.42931694-95
TACCGTG346450.017.370067
TAGACGG240300.017.295485
CTAGACA437450.016.4281624
TACACCG321600.016.0986255
CACTGAG696250.016.0790217
ACTGAGC639800.016.0277448
CCTAGAC409800.015.8791453
Produced by FastQC (version 0.11.5)